University of Washington Viral Vector Core Lab

1. Center Institution(s): University of Washington
2. Title of core facility or other resource: Viral Vector Core Lab
3. Contact person, email address, phone number:
   Jeffrey S. Chamberlain JSC5@u.washington.edu
   James M. Allen allenjm@u.washington.edu
4. Currently available or expected date of availability: Currently available
5. Requirements for use (by collaboration or fee-for-service, application required, etc.):
   Fee for service; application required
6. Brief description of services or resources (a paragraph or two)

   The Seattle MDCRC Viral Vector Core Lab is focused on generating recombinant adeno-associated viral vectors encapsidated in the AAV6 capsid [ Blankinship MJ, Gregorevic P, Allen JM, Harper SQ, Harper H, Halbert CL, Miller DA, Chamberlain JS. Efficient transduction of skeletal muscle using vectors based on adeno-associated virus serotype 6. Mol Ther. 2004;10:671-8 ]. We are also able to generate stocks of lentiviral vectors using the 4 plasmid co-transfection system developed by Luigi Naldini [e.g. Li S, Kimura E, Fall BM, Reyes M, Angello JC, Welikson R, Hauschka SD, Chamberlain JS: Stable transduction of myogenic cells with lentiviral vectors expressing a minidystrophin. Gene Ther. 2005; 12:1099-108] . The Core can also make certain plasmids related to these vectors available, although some may require signed material transfer agreements from the original sources. Due to the labor and time intensive nature of the methods needed to prepare stocks of recombinant viral vectors, we must review applications for the use of these services. Requested vectors must be used for muscular dystrophy research. This Core lab is also available to send out plasmids, mice and other reagents generated in our Center, especially those generated in the Chamberlain lab. For details, please visit: http://depts.washington.edu/mdcrc/index.html.